Korean Journal of Gastrointestinal Endoscopy 2003;26(2): 79-83.
장결핵에서 중합효소 연쇄반응법의 유용성
이태규·김영환·장우임·전은정·김진일·박수헌·한준열·김재광·정규원·선희식
가톨릭대학교 의과대학 내과학교실
The Diagnostic Value of Polymerase Chain Reaction in Intestinal Tuberculosis
Tae Kyu Lee, M.D., Young Hwan Kim, M.D., U Im Chang, M.D., Eun Jung Jun, M.D.,
Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
Abstract
Background/Aims: It is quite difficult to differentiate intestinal tuberculosis from Crohn's disease because of the similarities of their clinical and pathological features and low detection rate of acid fast bacilli. The diagnostic value of PCR has been studied in pulmonary tuberculosis, tuberculous pleuritis and meningitis, but few reports were made in cases of intestinal tuberculosis. The aim of this study is to evaluate the diagnostic value of PCR in intestinal tuberculosis. Methods: The subjects, a total of 70 cases are composed of clinically diagnosed intestinal tuberculosis, Crohn's disease and intestinal Behcet's disease. We performed PCR with paraffin-embedded intestinal tissue to detect the DNA of Mycobacterium Tuberculosis and the data was analyzed. Results: The positive rate of PCR for Mycobacterium Tuberculosis was 9.8% (4/41) in intestinal tuberculosis, 0% (0/29) in Crohn's disease, and 0% (0/3) in intestinal Behcet's disease. The granulomas were not found in 50% (2/4) of the PCR positive intestinal tuberculosis cases. Conclusions: We were not able to find evidences to prove the excellent value of PCR assay in making differential diagnosis of intestinal tuberculosis from other granulomatous diseases. But it might be helpful in detecting cases of intestinal tuberculosis which were not pathologically or microbiologically confirmed. (Korean J Gastrointest Endosc 2003;26:7983)
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