Comparative Cholangiocytotoxicity between Ioxithalamate and Iopromide with Short-term Exposure

Korean J Gastrointest Endosc > Volume 32(2); 2006 > Article

Korean Journal of Gastrointestinal Endoscopy 2006;32(2): 101-108.

단기간의 조영제 노출에 따른 Ioxithalamate와 Iopromide의 담낭 상피 세포의 독성 비교

주영민·이성구·김명환·서동완·이상수·차재명·최은광·정석원

울산대학교 의과대학 소화기내과학교실

Comparative Cholangiocytotoxicity between Ioxithalamate and Iopromide with Short-term Exposure

Young Min Ju, M.D., Sung Koo Lee, M.D., Myung Hwan Kim, M.D., Dong Wan Seo, M.D., Sang Soo Lee, M.D., Jae Myung Cha, M.D., Eun Kwang Choi, M.D. and Seok Won Jung, M.D.

Division of Gastroenterology, Ulsan University College of Medicine, Asan Medical Center, Seoul, Korea

Abstract

Background/Aims:
In a previous study examining long- term exposure to contrast media, high-osmolar ionic contrast media were reported to be more cytotoxic to gallbladder epithelial cells than low-osmolar nonionic contrast media. However, biliary epithelial cells are rarely exposed to contrast media for such long periods in clinical practice. This study compared the cytotoxicity of two types of contrast media to gallbladder epithelial cells exposed for a short-term.
Methods:
Ioxithalamate and iopromide were tested, and dog gallbladder epithelial cells were used as the test cells. The cells were exposed to the two contrast agents with increasing iodine concentration and osmolality for 30 minutes. The number of cells, aneuploidy and supernatant LDH activities were measured each day.
Results:
The growth of cells exposed to the two contrast media was significantly reduced but there was no difference between the two contrast media at the same iodine concentration. The level of cell lysis measured by the supernatant LDH activities before the triton X-100 treatment was similar with the two contrast media. No aneuploidy fraction was detected in any of the cell groups treated with the two contrast media for 5 days.
Conclusions:
In contrast to previous results, high-osmolar ionic and low-osmolar nonionic contrast media with short- term exposure were found to have a similar cytotoxicity to biliary epithelial cells. (Korean J Gastrointest Endosc 2006;32:101⁣108)

Key Words: Contrast media, Gallbladder epithelial cells, ERCP, Cytotoxicity

주요어: 조영제, 담낭 상피 세포, 내시경 역행성 담췌관 조영술, 세포 독성