Mucosal incision-assisted biopsy versus endoscopic ultrasound-assisted tissue acquisition for subepithelial lesions: a systematic review and meta-analysis

Article information

Clin Endosc. 2022;55(5):615-625

Publication date (electronic) : 2022 August 4

doi : https://doi.org/10.5946/ce.2022.133

Suprabhat Giri¹, Shivaraj Afzalpurkar², Sumaswi Angadi¹, Sridhar Sundaram^,3

¹Nizam's Institute of Medical Sciences, Hyderabad, India

²Institute of Gastrosciences and Liver, Apollo Multispecialty Hospital, Kolkata, India

³Department of Digestive Diseases and Clinical Nutrition, Tata Memorial Hospital, Mumbai, India

Correspondence: Sridhar Sundaram Department of Digestive Disease and Clinical Nutrition, TATA Memorial Hospital, Homi Bhabha National Institute, Dr. E Borges Road, Parel, Mumbai 400012, India E-mail: drsridharsundaram@gmail.com

Received 2022 April 22; Revised 2022 June 10; Accepted 2022 June 16.

Abstract

INTRODUCTION

Subepithelial lesions (SELs) of the gastrointestinal (GI) tract arise from the muscularis mucosa, submucosa, or muscularis propria. SELs, although most commonly are incidental findings on endoscopy, can rarely present with bleeding, dysphagia, gastric outlet obstruction, and metastasis based on size, nature of the lesion, and location in the GI tract.1 The detection rate of SELs has increased recently owing to the increased use of screening endoscopies and the advent of technology.2 Although most SELs are benign, 15% can be malignant at presentation.3 Hence, appropriate identification and characterization of these lesions are of utmost importance.

Although SELs are routinely identified on endoscopy, endoscopic ultrasonography (EUS) is the first-line modality for characterizing SELs as it provides information regarding the layer of origin, intramural/extramural location, size and shape, echogenicity, vascularity, and associated lymphadenopathy. The initial mode of tissue acquisition (TA) for diagnosis was made using jumbo biopsy forceps with the bite-on-bite technique rather than the standard biopsy forceps. In a retrospective analysis, TA with jumbo biopsy forceps had a diagnostic yield of 60%, with a better yield compared to that of the EUS fine-needle aspiration (FNA) in lesions arising from the submucosal layer (65.1% vs. 37.5%) than the muscularis propria layer (40% vs. 57.1%), but with a higher risk of bleeding when a biopsy was performed on lesions arising from the fourth layer.4

With increasing availability, EUS-guided TA using FNA or fine-needle biopsy (FNB) is currently the most commonly employed method. However, the diagnostic yield of EUS-FNA is affected by the availability of rapid on-site evaluation by a cytopathologist and the size of the lesion, with lesions less than 2 cm having a poor diagnostic yield compared to that of the larger lesions.5,6 TA using EUS-FNB obviates the need for rapid on-site evaluation, requires fewer passes, and preserves tissue architecture. However, previous meta-analyses comparing TA using FNA and FNB needles have reported conflicting results.7-9

Since the original description of the technique by Yokohata et al.,10 mucosal incision-assisted biopsy (MIAB) or single incision with a needle knife has gained importance as an alternative method of TA. A mucosal incision line was chosen for this technique, and saline with 0.001% epinephrine was injected submucosally. A mucosal incision was made using an electrosurgical knife. After submucosal dissection, a biopsy of the exposed SEL was performed using conventional biopsy forceps, followed by closure of the mucosal incision with endoclips.11 In a meta-analysis, Dhaliwal et al.12 showed a high pooled diagnostic yield of MIAB and relatively shorter operating time. An MIAB variant, endoscopic submucosal dissection-assisted deep biopsy has shown a pooled diagnostic rate of 95% with a very low rate of adverse events (AEs).13 Given the high diagnostic yield of MIAB, it can serve as an alternative to EUS-guided TA for the diagnosis of upper GI SELs with minimal complications. Hence, the present systematic review and meta-analysis aimed to compare EUS-FNA/B and MIAB for the optimal method of TA.

METHODS

RESULTS

Study characteristics and quality

Total 825 records were identified in the search, and 536 were screened after removing duplicates. Figure 1 shows the PRISMA flowchart of the article selection process. Seven studies17-23 were included in the meta-analysis. Table 1 summarizes the characteristics of the included studies. The majority of the studies were from Asia,17-21 one study was from Europe,22 and another multicenter study involved centers in North America and Europe.23 Three studies were prospective,18,20,21 three were RCTs1,19,23 and one was retrospective.21 The SEL was located in the stomach in most of the studies,17-19,21 while three studies20,22,23 included lesions in the esophagus and duodenum along with the stomach. The pooled mean age of the population was 61.1±12.1 years. With respect to EUS-guided TA, four studies used FNA needles,17-19,22 two used FNB needles,20,23 and one used both.21 Among the RCTs, only one had a low risk of bias,23 whereas the other two had a moderate risk of bias (Fig. 2A).19,22 Among the non-randomized studies, one study had a low risk of bias,18 two had a moderate risk of bias,20,21 and one had a high risk of bias (Fig. 2B).17

Fig. 1.

Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) flowchart for the study selection process.

Table 1.

Study characteristics of included studies

Fig. 2.

Risk of bias assessment for randomized controlled trials (A) and non-randomized studies (B).

Outcomes

1) Technical success

All seven studies17-23 with 520 patients reported the technical success of both procedures. Both MIAB and EUS-guided TA had comparable technical success rates (RR, 0.94; 95% confidence interval [CI], 0.87–1.03; I²=81%), with significant heterogeneity among the studies (Fig. 3). A sensitivity analysis using only RCTs19,22,23 also showed a comparable technical success between the two modalities (RR, 1.00; 95% CI, 0.94–1.06; I²=0%).

Fig. 3.

Forest plot comparing the technical success between mucosal incision-assisted biopsy and EUS-guided tissue acquisition. EUS, endoscopic ultrasound; MIAB, mucosal incision-assisted biopsy; SD, standard deviation; IV, intravenous; CI, confidence interval.

2) Positive diagnostic yield

All seven studies17-23 reported the outcome of diagnosis based on histology. The pooled rate of diagnostic yield with MIAB was 90.7% (95% CI, 84.7–96.7; I²=71.8%), which was significantly higher than that of the EUS-guided TA 72.1% (95% CI, 60.7–83.4; I²=78.5%). The pooled rate of diagnostic yields with EUS-FNA and EUS-FNB were 73.8% (95% CI, 58.4–89.3; I²=85.1%) and 77.7% (95% CI, 59.8–95.7; I²=78.7%), respectively. Use of EUS for TA from SELs was associated with a lower diagnostic yield compared to that of MIAB (RR, 0.83; 95% CI, 0.71–0.98; I²=67%), with significant heterogeneity among the studies (Fig. 4). Meta-regression to assess the source of heterogeneity could not be performed because the number of studies included in the analysis was less than ten.

Fig. 4.

Forest plot comparing the diagnostic yield between mucosal incision-assisted biopsy and EUS-guided tissue acquisition. EUS, endoscopic ultrasound; MIAB, mucosal incision-assisted biopsy; M-H, Mantel-Haenszel; CI, confidence interval.

Heterogeneity can be due to differences in the needle used, size of the mass lesion, and location of the lesion. Hence, a subgroup analysis was conducted to compare the diagnostic rates of EUS-FNB and MIAB. Both methods were comparable in achieving a diagnosis without any heterogeneity (RR, 0.93; 95% CI, 0.83–1.04; I²=0%) (Fig. 5A). Comparing both techniques with respect to the lesion size, MIAB was better than EUS-guided TA without any heterogeneity (RR, 0.75; 95% CI, 0.63–0.89; I²=0%) (Fig. 5B). On sensitivity analysis of only RCTs,19,22,23 the diagnostic yield was comparable between the two modalities (RR, 0.91; 95% CI, 0.77–1.07; I²=30%). Analyzing available data of only gastric SELs,17-21 the diagnostic yield was lower with EUS than with MIAB (RR, 0.77; 95% CI, 0.62–0.97; I²=71%) (Fig. 5C). However, on subgroup analysis of three studies19-21 based on the location of the lesion in stomach, there was no difference in the diagnostic yield between lesions in upper stomach (RR, 0.89; 95% CI, 0.79–1.01; I²=0%), middle stomach (RR, 0.88; 95% CI, 0.69–1.11; I²=0%), and lower stomach (RR, 0.72; 95% CI, 0.49–1.06; I²=15%).

Fig. 5.

Forest plot comparing the diagnostic yield between mucosal incision-assisted biopsy and EUS-guided tissue acquisition for subgroups. (A) Use of EUS-FNB. (B) Size of lesion <20 mm. (C) Gastric subepithelial lesions. EUS, endoscopic ultrasound; FNB, fine-needle biopsy; MIAB, mucosal incision-assisted biopsy; M-H, Mantel-Haenszel; CI, confidence interval.

3) Procedural time

Overall, five studies17-19,21,23 including 418 patients reported differences in procedural time with both techniques. There was no significant difference in the procedural time, although there was significant heterogeneity (mean difference [MD], –4.53 seconds; 95% CI, –22.38 to 13.31; I²=99%) (Fig. 6). A sensitivity analysis pooling data from only RCTs19,23 showed no difference in the procedural time (MD, –5.81 seconds; 95% CI, –14.53 to 2.91; I²=89%).

Fig. 6.

Forest plot comparing the procedural time between mucosal incision-assisted biopsy and EUS-guided tissue acquisition. EUS, endoscopic ultrasound; MIAB, mucosal incision-assisted biopsy; M-H, Mantel-Haenszel; CI, confidence interval.

4) Procedural complications

Among the seven included studies,17-23 six did not report any AE associated with either procedure. In the study by Sanaei et al.,23 the rate of AEs was 7.69% (95% CI, 2.13–24.14) in the EUS-FNB group as compared to 10% (95% CI, 3.45–25.62) in the MIAB group (p>0.99). In the EUS-group, two patients had abdominal pain, whereas in the MIAB group, one had pain and two developed delayed bleeding within six days after the procedure. One of the bleeding episodes was self-limiting, whereas the other required arterial embolization.

5) Assessment of publication bias and leave-one-out analysis

Visual assessment of the funnel plot (Supplementary Fig. 1) and Egger’s test (Supplementary Table 1) showed the presence of publications for both technical success and diagnostic accuracy, but not for procedural time. A leave-one-out meta-analysis (Supplementary Fig. 2) showed a significant change in procedural time (Supplementary Fig. 2C). With the exclusion of the study by Jung et al.,17 EUS-guided TA was associated with a significantly shorter procedure duration than MIAB (MD, –12.34 seconds; 95% CI, –21.05 to –3.64; I²=91%). Table 2 summarizes findings with confidence in the evidence.

Table 2.

Summary of findings and grade of evidence

DISCUSSION

The present meta-analysis attests to the role of MIAB as an alternative to EUS-guided TA in upper GI SELs. The analysis showed a similar technical success rate with EUS-guided TA and MIAB (RR, 0.96; 95% CI, 0.89–1.04), but a lower rate of diagnostic yield with EUS-guided TA (RR, 0.83; 95% CI, 0.71–0.98). On subgroup analysis, EUS-FNB was comparable to MIAB with respect to the diagnostic yield (RR, 0.93; 95% CI, 0.83–1.04). However, the diagnostic yield of EUS-guided TA was lower than that of MIAB for diagnosing lesions less than 20 mm (RR, 0.75; 95% CI, 0.63–0.89). Both techniques were associated with significantly low AE rates. Although the procedural time was similar in both methods on overall analysis (MD, –5.81 seconds; 95% CI, –14.53 to 2.91), the study by Jung et al.17 was a significant outlier, and with its exclusion, the mean procedural time was lower with EUS (MD, –12.34 seconds; 95% CI, –21.05 to –3.64).

The current guidelines by the European Society of Gastrointestinal Endoscopy (ESGE) recommend tissue diagnosis for all SELs with features suggestive of gastrointestinal stromal tumor (GIST), size >20 mm, associated high-risk stigmata on EUS, or prior to surgical resection.24 The European Society for Medical Oncology25 and the Japanese GIST Guideline Subcommittee26 recommend resection of GIST, even those <20 mm. Hence, tissue sampling for pathological and immunohistochemical analyses is a critical step in managing SELs. The ESGE recommends using either MIAB or EUS-guided TA for sampling SELs >20 mm in size. For SELs <20 mm in size, the ESGE recommends MIAB as the first choice, followed by EUS-guided TA.24 Dhaliwal et al.,12 in a meta-analysis on the outcome of MIAB for upper GI SELs, reported an overall pooled diagnostic yield of 89% without any heterogeneity. In the current meta-analysis, MIAB was associated with a higher chance of diagnosis than EUS-guided TA. Hence, the utility of MIAB in the diagnosis of SELs needs to be explored in larger studies.

Meta-analyses comparing TA with SELs using FNA and FNB needles have reported conflicting results. Two meta-analyses reported better diagnostic accuracy with FNB,7,8 while another meta-analysis reported no difference in accuracy with FNA/FNB or based on the choice of needle employed.9 The pooled rates of diagnostic yield in the present analysis with MIAB, EUS-FNB, and EUS-FNA were 90.7% (95% CI, 84.7–96.7), 77.7% (95% CI, 59.8–95.7), and 73.8% (95% CI, 58.4–89.3), respectively. In subgroup analysis comparing the diagnostic rate of EUS-FNB with MIAB in the present study, both methods were comparable in achieving a diagnosis without any heterogeneity (RR, 0.93; 95% CI, 0.83–1.04). Considering the overall quality of the tissue obtained, both EUS-FNB and MIAB may be superior to EUS-FNA for the histological diagnosis of SELs.

In a study analyzing the factors influencing the diagnostic yield of EUS-FNA for SELs, the diagnostic accuracy was 50% for lesions <20 mm and 91.6% for those >20 mm.6 Akahoshi et al.5 reported a diagnostic rate of 71% for lesions <20 mm, 86% for those between 20 mm and 40 mm, and 100% for those >40 mm. This is mainly because the smaller size of the lesion makes it difficult to target using EUS-guided TA techniques. In the present analysis, the diagnostic yield of EUS-guided TA was lower than that of MIAB for small SELs <20 mm (RR, 0.75; 95% CI, 0.63–0.89). The low diagnostic yield of EUS-guided TA in the study by Kobara et al.18 may be attributed to the fact that approximately 74% of the SEL were smaller than 2 cm. Hence, for SELs <20 mm, MIAB may be considered the preferred option over EUS. However, this rule has a caveat. Kim27 proposed a classification method to determine whether GISTs were predominantly intramural or extramural. Those with predominant extramural components (types III and IV) may not be adequately sampled using MIAB, and EUS-guided TA may be the preferred modality in these situations.

The present analysis showed no significant difference in the procedural time (MD, –4.53 seconds; 95% CI, –22.38 to 13.31), although there was significant heterogeneity. Therefore, these findings should be interpreted with caution. In a leave-one-out meta-analysis, after excluding the influential study by Jung et al.,17 the procedural timing was significantly shorter with EUS. Thus, MIAB may be associated with longer procedure duration than EUS.

Dhaliwal et al.12 reported a pooled clinically significant post-procedural bleeding rate of 5.03% (95% CI, 0.4–12.9, I²=57.43%) with MIAB, but no perforation. In the present analysis, six17-22 of the seven included studies reported no AEs with either of the procedures. Only the study by Sanaei et al.23 reported AEs, such as abdominal pain and bleeding associated with both techniques without any significant difference (p=1.0). This indicates the comparable safety of the two procedures. In terms of other available techniques for tissue diagnosis of SELs, Facciorusso et al.28 compared EUS-FNB with bite-on-bite biopsy using jumbo forceps. The sample adequacy and diagnostic accuracy were significantly higher with EUS-FNB (94.1% vs. 77.5% and 89.3% vs. 67.1%, respectively), with a lower bleeding rate (6.6% vs. 29.1%). However, there are no recent comparative studies that evaluated the outcome of MIAB with jumbo-forceps biopsy.

The findings of this study are important for several reasons. First, the overall diagnostic yield with MIAB is comparable to that of EUS-guided TA and is better for lesions <20 mm in size. MIAB can be performed during routine endoscopy and no advanced equipment is required. Second, lesion size does not affect the diagnostic yield of MIAB, while needle passage and aspiration of small-sized SELs may be challenging with EUS. Third, MIAB can be easily performed regardless of the anatomic location of the lesion in the stomach, provided a reasonable bulge is visualized endoscopically (type I and II according to Kim’s classification). In contrast, EUS-guided TA, especially FNA, can have a higher failure rate when the SEL is in the cardia or fundus because the stiff device has difficulty accessing these areas.17 To address the suboptimal diagnostic yield of EUS-FNA, a study was conducted using a forward-viewing echoendoscope, which showed a complete histological assessment in 93.4% of patients.29 However, a subsequent RCT30 reported comparable rates of histologic diagnosis between forward- and oblique-viewing echoendoscopes (80.5% vs. 73.2%; p=0.453).

There were a few limitations in the study, most inherent to any meta-analysis, which warrant further discussion. First, most of the studies were from a single center and two were retrospective. Second, the RCTs included in the analysis were underpowered to demonstrate a reasonable difference. Third, there was moderate to considerable heterogeneity in the studies with respect to the type of needle used and size of the mass lesions. Moreover, the definitions for procedural time varied among the studies, and one study did not define procedural time. Finally, economic considerations regarding the impact of sampling by MIAB or EUS-guided TA were beyond the scope of the current meta-analysis.

In conclusion, MIAB and EUS-guided TA have comparable technical success, diagnostic accuracy, and procedural time, but with significant heterogeneity. However, MIAB was better than EUS-guided TA for small lesions without heterogeneity. MIAB is an alternative to EUS-guided TA in clinical practice and may be the procedure of choice for SELs <20 mm in size. In centers where EUS expertise is unavailable, MIAB is an easy and safe alternative for EUS-guided TA. Large multicenter trials are the need of the hour to validate the findings of this meta-analysis.

Supplementary Material

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